Table 1. Gas Chromatographic Columnsa Used in Analytical and Preparative GC and in Coupled Gas Chromatography-Mass Spectrometry. |
Analytical GC: Instrument: Hewlett-Packard, model 5830.
Polar GC column 1: Fused silica; length = 46 m, ID = 0.35 mm; OV-351, df = 0.59 wm. Temperature program: 50o C for 5 min, 5oC/min to 200oC, then isothermal for 20 min. Preparative GC: Instrument: Carlo Erba, model 4160. Polar GC column 2: Fused silica; length = 24 m, ID = 0.35 mm; OV-351, df = 0.5 µm. Temperature program: 5OoC for 2 min, 5oC/min to 220oC, then isothermal for 20 min. Mobile phase: N2 at 30 cm/sec. Nonpolar GC column 3: Fused silica; length = 26 m, ID = 0.32 mm; SE-33 cross-linked, df = 0.5 µm. Temperature program: 80oC for 2 min, 5oC/min to 220oC, then isothermal for 20 min. Mobile phase: N2 at 30 cm/sec. Analytical GC-MS: Instrument: Finnigan, model 402l. Polar GC column 4: Fused silica; length = 23 m, ID = 0.20 mm; OV-35l, df = 0.5 µm. Temperature program: 50oC for 4 min, 8oC/min to 200oC, then isothermal for 10 min. Mobile phase: He at 20 cm/sec. Nonpolar GC column 5: Fused silica; length = 12.5 m, ID = 0.20 mm; SE-54 cross-linked. Temperature program: 80oC for 3 min, 5oC/min to 220oC, then isothermal for 10 min. Mobile phase: He at 20 cm/sec. Quantitative GC-MS: Instrument: Finnigan. model 4021. Polar GC column 6: Fused silica; length = 25 m, ID = 0.15 mm; Superox FA, df = 0.3 µm. Temperature program: 50oC for 4 min, 8oC/min to 200oC, then isothermal for 10 min. Mobile phase: He at 20 cm/sec. |
---|
aGC column 5, Hewlett-Packard, other GC columns prepared at Department of Chemical Ecology. Göteborg University. |
Table 2. Attraction of female Pityogenes chalcographus in the laboratory bioassay to mixtures of gas chromatographic fractions (polar GC column 2, Table 1) of airborne volatile extracts from males boring in Norway spruce logs. | ||
Stimulus | % Females respondingb ( N = 30-40 ) |
95% CIc |
---|---|---|
Fractions tested March 15, 1983 ( cf. Figure 1A ) | ||
1 + 2 + 3 + 4 + 5 = total blend | 65.0a | 49.5 - 77.9 |
Total blend - 1 | 37.5b | 24.2 - 53.0 |
Total blend - 2 | 60.0a | 44.6 - 73.7 |
Total blend - 3 | 0.0d | 0.0 - 12.8 |
Total blend - 4 | 63.3a | 45.5 - 78.1 |
Total blend - 5 | 12.5c | 5.5 - 26.1 |
3 (natural chalcogran) | 13.3c | 5.3 - 29.7 |
Fractions tested April 23, 1983 (cf. Figure 1A) | ||
1 + CH + 5A + 5B + 5C + 5D + 5E = blend | ||
Blend - 5A | 63.3a | 45.5 - 78.1 |
Blend - 5B | 60.0a | 42.3 - 75.4 |
Blend - 5C | 50.0a | 33.2 - 66.8 |
Blend - 5D | 46.7a | 30.2 - 63.9 |
Blend - 5E | 10.0b | 3.5 - 25.6 |
CHd + 5E | 46.7a | 30.2 - 63.9 |
Fractions tested May 10, 1983 (cf. Figure 1A) | ||
CHd + 5Ea + 5Eb + 5Ec + 5Ed + 5Ee = blend | 50.0a | 33.2 - 66.9 |
Blend - 5Ea | 50.0a | 33.2 - 66.9 |
Blend - 5Eb | 46.7a | 30.2 - 63.9 |
Blend - 5Ec | 46.7a | 30.2 - 63.9 |
Blend - 5Ed | 50.0a | 33.2 - 66.9 |
Blend - 5Ee | 40.0a | 24.6 - 57.7 |
Fractions tested May 22, 1983 (cf. Figure 1A) | ||
CHd + 5Ea | 56.7a | 39.2 - 72.6 |
CHd + 5Eb | 70.0a | 52.1 - 83.3 |
CHd + 5Ec | 3.3b | 0.6 - 16.7 |
CHd + 5Ed | 6.7b | 1.8 - 21.3 |
CHd + 5Ee | 10.0b | 3.5 - 25.6 |
Table 3. Attraction of female Pityogenes chalcographus in the laboratory bioassay to mixtures of gas chromatographic fractions (nonpolar GC column 3, Table 1) of airborne volatile extracts from males boring in Norway spruce logs. | ||
Stimulus | % Females respondingb ( N = 30 ) |
95% CIc |
---|---|---|
Fractions tested April 3, 1984 ( cf. Figure 1B ) | ||
CHd + 5E:I + 5E:II + 5E:III = blend | 60.0a | 42.3 - 75.4 |
Blend - 5E:I | 16.7b | 0.7 - 33.6 |
Blend - 5E:II | 53.3a | 36.1 - 69.8 |
Blend - 5E:III | 53.3a | 36.1 - 69.8 |
CHd + 5E:I | 40.0a | 24.6 - 57.7 |
CHd + 5E:II | 13.3b | 0.5 - 29.7 |
CHd + 5E:III | 13.3b | 0.5 - 29.7 |
J. A. BYERS1, G. BIRGERSSON2, J. LÖFQVIST1, M. APPLEGREN2, and G. BERGSTRÖM2 1Department of Animal Ecology, Lund University, SE-223 62 Lund, Sweden 1Present address: 2Chemical Ecology, Göteborg University, SE-405 30 Göteborg, Sweden |
---|